Identification of an ATP-sensitive potassium channel in mitochondria.

ABSTRACT: Mitochondria provide chemical energy for endoergonic reactions in the form of ATP, and their activity must meet cellular energy requirements, but the mechanisms that link organelle performance to ATP levels are poorly understood. Here we confirm the existence of a protein complex localized in mitochondria that mediates ATP-dependent potassium currents (that is, mitoK_ATP). We show that-similar to their plasma membrane counterparts-mitoK_ATP channels are composed of pore-forming and ATP-binding subunits, which we term MITOK and MITOSUR, respectively. In vitro reconstitution of MITOK together with MITOSUR recapitulates the main properties of mitoK_ATP. Overexpression of MITOK triggers marked organelle swelling, whereas the genetic ablation of this subunit causes

SUBMITTER: Paggio A 

PROVIDER: S-EPMC6726485 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature