Establishment of a novel experimental model for muscle-invasive bladder cancer using a dog bladder cancer organoid culture.

ABSTRACT: In human and dogs, bladder cancer (BC) is the most common neoplasm affecting the urinary tract. Dog BC resembles human muscle-invasive BC in histopathological characteristics and gene expression profiles, and could be an important research model for this disease. Cancer patient-derived organoid culture can recapitulate organ structures and maintains the gene expression profiles of original tumor tissues. In a previous study, we generated dog prostate cancer organoids using urine samples, however dog BC organoids had never been produced. Therefore we aimed to generate dog BC organoids using urine samples and check their histopathological characteristics, drug sensitivity, and gene expression profiles. Organoids from individual BC dogs were successfully generated, expressed urothelial cell m

SUBMITTER: Elbadawy M 

PROVIDER: S-EPMC6726682 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature