Lipid peroxidation increases hydrogen peroxide permeability leading to cell death in cancer cell lines that lack mtDNA.

ABSTRACT: 4-Hydroxynonenal (HNE) is an important product of plasma membrane lipid peroxidation, which is a cause of cell and tissue injury. Mitochondrial DNA (mtDNA)-depleted ρ⁰ cells were established using human cervical cancer and oral squamous cell carcinoma cell lines. We investigated the effect of reactive oxygen species in ρ⁰ cells, especially the mechanism of hydrogen peroxide (H₂ O₂ )-mediated cell death. These cell were subjected to high oxidative stress and, compared with their parental cells, showed greater sensitivity to H₂ O₂ and high lipid peroxidation. Upregulation of HNE in the plasma membrane was observed prior to the increase in intracellular H₂ O₂ . The amount of oxidized lipid present changed

SUBMITTER: Tomita K 

PROVIDER: S-EPMC6726706 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature