FAM83D directs protein kinase CK1α to the mitotic spindle for proper spindle positioning.

ABSTRACT: The concerted action of many protein kinases helps orchestrate the error-free progression through mitosis of mammalian cells. The roles and regulation of some prominent mitotic kinases, such as cyclin-dependent kinases, are well established. However, these and other known mitotic kinases alone cannot account for the extent of protein phosphorylation that has been reported during mammalian mitosis. Here we demonstrate that CK1α, of the casein kinase 1 family of protein kinases, localises to the spindle and is required for proper spindle positioning and timely cell division. CK1α is recruited to the spindle by FAM83D, and cells devoid of FAM83D, or those harbouring CK1α-binding-deficient FAM83D^F283A/F283A knockin mutations, display pronounced spindle positioning defects, and a pro

SUBMITTER: Fulcher LJ 

PROVIDER: S-EPMC6726907 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature