Equine urinary N-acetyl-β-D-glucosaminidase assay validation and correlation with other markers of kidney injury.

ABSTRACT: Detection of equine acute kidney injury (AKI) is hindered by limited markers of early renal damage in horses. N-acetyl-β-D-glucosaminidase (NAG), a lysosomal enzyme in renal tubular epithelium released into urine during tubular insult, has shown promise for early identification of AKI in humans and other species. We validated an assay for NAG in equine urine and measured urinary NAG in 7 azotemic and 7 non-azotemic client-owned adult horses. The enzymatic NAG assay was validated using within- and between-run coefficients of variation (CVs), recovery following standard addition, and linearity of dilution. Intra- and inter-run CVs (21% and 3.2%, respectively), average recovery following standard addition (99-109%), and linearity under serial dilution (R² = 0.997) were satis

SUBMITTER: Bayless RL 

PROVIDER: S-EPMC6727119 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature