Development of a multiplex PCR to detect and discriminate porcine circoviruses in clinical specimens.

ABSTRACT:

Background

A diagnostic method to simultaneously detect and discriminate porcine circovirus type 1 (PCV1), porcine circovirus type 2 (PCV2) and porcine circovirus type 3 (PCV3) in clinical specimens is imperative for the differential diagnosis and monitoring and control of PCVs in the field.

Methods

Three primer pairs were designed and used to develop a multiplex PCR assay. And 286 samples from 8 farms in Hubei province were tested by the developed multiplex PCR assay to demonstrate the accuracy.

Results

Each of target genes of PCV1, PCV2 and PCV3 was amplified using the designed primers, while no other porcine viruses genes were detected. The limit of detection of the assay was 10 copies/μL of PCV1, PCV2 OR PCV3. The results of the tissue samples detection showed tha

SUBMITTER: Yang K 

PROVIDER: S-EPMC6727504 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature