Fission yeast TRP channel Pkd2p localizes to the cleavage furrow and regulates cell separation during cytokinesis.

ABSTRACT: Force plays a central role in separating daughter cells during cytokinesis, the last stage of cell division. However, the mechanism of force sensing during cytokinesis remains unknown. Here we discovered that Pkd2p, a putative force-sensing transient receptor potential channel, localizes to the cleavage furrow during cytokinesis of the fission yeast, Schizosaccharomyces pombe. Pkd2p, whose human homologues are associated with autosomal polycystic kidney disease, is an essential protein whose localization depends on the contractile ring and the secretory pathway. We identified and characterized a novel pkd2 mutant pkd2-81KD. The pkd2 mutant cells show signs of osmotic stress, including temporary shrinking, paused turnover of the cytoskeletal structures, and hyper

SUBMITTER: Morris Z 

PROVIDER: S-EPMC6727746 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature