Anchoring of actin to the plasma membrane enables tension production in the fission yeast cytokinetic ring.

ABSTRACT: The cytokinetic ring generates tensile force that drives cell division, but how tension emerges from the relatively disordered ring organization remains unclear. Long ago, a musclelike sliding filament mechanism was proposed, but evidence for sarcomeric order is lacking. Here we present quantitative evidence that in fission yeast, ring tension originates from barbed-end anchoring of actin filaments to the plasma membrane, providing resistance to myosin forces that enables filaments to develop tension. The role of anchoring was highlighted by experiments on isolated fission yeast rings, where sections of ring became unanchored from the membrane and shortened ∼30-fold faster than normal. The dramatically elevated constriction rates are unexplained. Here we present a molecularly explicit simu

SUBMITTER: Wang S 

PROVIDER: S-EPMC6727776 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature