Cohesin Impedes Heterochromatin Assembly in Fission Yeast Cells Lacking Pds5.

ABSTRACT: The fission yeast Schizosaccharomyces pombe is a powerful genetic model system for uncovering fundamental principles of heterochromatin assembly and epigenetic inheritance of chromatin states. Heterochromatin defined by histone H3 lysine 9 methylation and HP1 proteins coats large chromosomal domains at centromeres, telomeres, and the mating-type (mat) locus. Although genetic and biochemical studies have provided valuable insights into heterochromatin assembly, many key mechanistic details remain unclear. Here, we use a sensitized reporter system at the mat locus to screen for factors affecting heterochromatic silencing. In addition to known components of heterochromatin assembly pathways, our screen identified eight new factors including the cohesin-associated protein

SUBMITTER: Folco HD 

PROVIDER: S-EPMC6727797 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature