Western Blotting with Solutions containing Nanoliter Volumes of Antibody.

ABSTRACT: Whether screening small mammal serum during antibody production or attempting to preserve a stock of precious antibody, this protocol's western blotting method using aliquots containing nanoliter volumes of antibody will benefit researchers. Time-tested western blotting workflows allowing separation and analysis of proteins are routinely utilized in clinical and laboratory settings. The necessity for relatively large quantities of antibody is a major limitation to this universal tool. This article provides a step-by-step protocol for detecting proteins of interest with solutions containing nanoliter volumes of antibody without altering the preceding gel electrophoresis and transfer methods. Important considerations, frequently encountered problems, and means of optimizing reproducibility a

SUBMITTER: Cruz Walma DA 

PROVIDER: S-EPMC6727981 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature