Characterizing micro-to-millisecond chemical exchange in nucleic acids using off-resonance R_1ρ relaxation dispersion.

ABSTRACT: This review describes off-resonance R_1ρ relaxation dispersion NMR methods for characterizing microsecond-to-millisecond chemical exchange in uniformly ¹³C/¹⁵N labeled nucleic acids in solution. The review opens with a historical account of key developments that formed the basis for modern R_1ρ techniques used to study chemical exchange in biomolecules. A vector model is then used to describe the R_1ρ relaxation dispersion experiment, and how the exchange contribution to relaxation varies with the amplitude and frequency offset of an applied spin-locking field, as well as the population, exchange rate, and differences in chemical shifts of two exchanging species. Mathematical treatment of chemical exchange based on the Bloch-McConnell equ

SUBMITTER: Rangadurai A 

PROVIDER: S-EPMC6727989 | biostudies-literature | 2019 Jun - Aug

REPOSITORIES: biostudies-literature