Distinct mechanisms of microRNA sorting into cancer cell-derived extracellular vesicle subtypes.

ABSTRACT: Extracellular vesicles (EVs) encompass a variety of vesicles secreted into the extracellular space. EVs have been implicated in promoting tumor metastasis, but the molecular composition of tumor-derived EV sub-types and the mechanisms by which molecules are sorted into EVs remain mostly unknown. We report the separation of two small EV sub-populations from a metastatic breast cancer cell line, with biochemical features consistent with different sub-cellular origins. These EV sub-types use different mechanisms of miRNA sorting (selective and non-selective), suggesting that sorting occurs via fundamentally distinct processes, possibly dependent on EV origin. Using biochemical and genetic tools, we identified the Lupus La protein as mediating sorting of selectively packaged miRNAs. We found t

SUBMITTER: Temoche-Diaz MM 

PROVIDER: S-EPMC6728143 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature