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High-throughput determination of the antigen specificities of T cell receptors in single cells.

ABSTRACT: We present tetramer-associated T-cell receptor sequencing (TetTCR-seq) to link T cell receptor (TCR) sequences to their cognate antigens in single cells at high throughput. Binding is determined using a library of DNA-barcoded antigen tetramers that is rapidly generated by in vitro transcription and translation. We applied TetTCR-seq to identify patterns in TCR cross-reactivity with cancer neoantigens and to rapidly isolate neoantigen-specific TCRs with no cross-reactivity to the wild-type antigen.

SUBMITTER: Zhang SQ 

PROVIDER: S-EPMC6728224 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature

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High-throughput determination of the antigen specificities of T cell receptors in single cells.

Zhang Shu-Qi SQ   Ma Ke-Yue KY   Schonnesen Alexandra A AA   Zhang Mingliang M   He Chenfeng C   Sun Eric E   Williams Chad M CM   Jia Weiping W   Jiang Ning N  

Nature biotechnology 20181112

We present tetramer-associated T-cell receptor sequencing (TetTCR-seq) to link T cell receptor (TCR) sequences to their cognate antigens in single cells at high throughput. Binding is determined using a library of DNA-barcoded antigen tetramers that is rapidly generated by in vitro transcription and translation. We applied TetTCR-seq to identify patterns in TCR cross-reactivity with cancer neoantigens and to rapidly isolate neoantigen-specific TCRs with no cross-reactivity to the wild-type antig  ...[more]

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