Biological Deep Temperature Imaging with Fluorescence Lifetime of Rare-Earth-Doped Ceramics Particles in the Second NIR Biological Window.

ABSTRACT: Contactless thermal imaging generally relies on mid-infrared cameras and fluorescence imaging with temperature-sensitive phosphors. Fluorescent thermometry in the near-infrared (NIR) region is an emerging technique for analysing deep biological tissues but still requires observation depth calibration. We present an NIR fluorescence time-gated imaging (TGI) thermometry technology based on fluorescence lifetime, an intrinsic fluorophore time constant unrelated to observation depth. Fluorophore used is NaYF₄ co-doped with Nd³⁺ and Yb³⁺ that emits fluorescence at 1000 nm. An agarose gel-based phantom with the fluorophore embedded at a 5-mm depth was covered by sheets of meat to vary the observation depth. The temperature was determined independently from depth

SUBMITTER: Chihara T 

PROVIDER: S-EPMC6728332 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature