A Preliminary Study: Human Fibroid Stro-1⁺/CD44⁺ Stem Cells Isolated From Uterine Fibroids Demonstrate Decreased DNA Repair and Genomic Integrity Compared to Adjacent Myometrial Stro-1⁺/CD44⁺ Cells.

ABSTRACT:

Context

Although uterine fibroids (UFs) continue to place a major burden on female reproductive health, the mechanisms behind their origin remain undetermined. Normal myometrial stem cells may be transformed into tumor-initiating stem cells, causing UFs, due to unknown causes of somatic mutations in MED12, found in up to 85% of sporadically formed UFs. It is well established in other tumor types that defective DNA repair increases the risk of such tumorigenic somatic mutations, mechanisms not yet studied in UFs.

Objective

To examine the putative cause(s) of this stem cell transformation, we analyzed DNA repair within stem cells from human UFs compared to those from adjacent myometrium to determine whether DNA repair in fibroid stem cells is compromised.

Design

Human f

SUBMITTER: Prusinski Fernung LE 

PROVIDER: S-EPMC6728578 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature