Detection of Giardia spp. with formalin/ether concentration in Brassica oleracea (cabbage) and Lactuca sativa (lettuce).

ABSTRACT: Methods to detect protozoa are needed for food safety monitoring. We evaluated protocols to recover Giardia spp. cysts in Brassica oleracea (cabbage) and Lactuca sativa (lettuce) and then detection was performed by concentrating with formalin/ether solutions and microscopy or immunofluorescence or DNA amplification via PCR. To evaluate this methodology, G. duodenalis cysts were inoculated in triplicate (10 cysts) in 35-g samples of lettuce and cabbage. The method obtaining the highest percentage of recovery in cabbage was sulfamic acid solution plus stirring with stomacher (47.7% ± 7.5). For lettuce, the best method was glycine solution plus stirring with stomacher (46.6% ± 5.3). Inter-observer agreement was of 0.99. Giardia was detected by amplifying spe

SUBMITTER: Hernandez-Arango N 

PROVIDER: S-EPMC6728726 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature