Molecular determinants of ligand selectivity in a vertebrate odorant receptor.

ABSTRACT: The identification of the chemical structure of an odorant by the vertebrate olfactory system is thought to occur through the combinatorial activity from multiple receptors, each tuned to recognize different chemical features. What are the molecular determinants underlying the selectivity of individual odorant receptors for their cognate ligands? To address this question, we performed molecular modeling and site-directed mutagenesis on the ligand-binding region of two orthologous amino acid odorant receptors belonging to the "C family" of G-protein-coupled receptors in goldfish and zebrafish. We identified the critical ligand-receptor interactions that afford ligand binding as well as selectivity for different amino acids. Moreover, predictions regarding binding pocket structure allowed us

SUBMITTER: Luu P 

PROVIDER: S-EPMC6730175 | biostudies-literature | 2004 Nov

REPOSITORIES: biostudies-literature