Triple-negative breast cancer cell line sensitivity to englerin A identifies a new, targetable subtype.

ABSTRACT:

Purpose

Triple-negative breast cancers (TNBCs) represent a heterogeneous group of tumors. The lack of targeted therapies combined with the inherently aggressive nature of TNBCs results in a higher relapse rate and poorer overall survival. We evaluated the heterogeneity of TNBC cell lines for TRPC channel expression and sensitivity to cation-disrupting drugs.

Methods

The TRPC1/4/5 agonist englerin A was used to identify a group of TNBC cell lines sensitive to TRPC1/4/5 activation and intracellular cation disruption. Quantitative RT-PCR, the sulforhodamine B assay, pharmacological inhibition, and siRNA-mediated knockdown approaches were employed. Epifluorescence imaging was performed to measure intracellular Ca²⁺ and Na⁺ levels. Mitochondrial membrane pot

SUBMITTER: Grant CV 

PROVIDER: S-EPMC6730645 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature