Cancer stem cell subpopulations in primary colon adenocarcinoma.

ABSTRACT:

Aims

The cancer stem cell concept proposes that tumor growth and recurrence is driven by a small population of cancer stem cells (CSCs). In this study we investigated the expression of induced-pluripotent stem cell (iPSC) markers and their localization in primary low-grade adenocarcinoma (LGCA) and high-grade adenocarcinoma (HGCA) and their patient-matched normal colon samples.

Materials and methods

Transcription and translation of iPSC markers OCT4, SOX2, NANOG, KLF4 and c-MYC were investigated using immunohistochemical (IHC) staining, RT-qPCR and in-situ hybridization (ISH).

Results

All five iPSC markers were detected at the transcriptional and translational levels. Protein abundance was found to be correlated with tumor grade. Based on their protein expression with

SUBMITTER: Munro MJ 

PROVIDER: S-EPMC6730900 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature