NOTCH Activation Promotes Valve Formation by Regulating the Endocardial Secretome.

ABSTRACT: The endocardium is a specialized endothelium that lines the inner surface of the heart. Functional studies in mice and zebrafish have established that the endocardium is a source of instructive signals for the development of cardiac structures, including the heart valves and chambers. Here, we characterized the NOTCH-dependent endocardial secretome by manipulating NOTCH activity in mouse embryonic endocardial cells (MEEC) followed by mass spectrometry-based proteomics. We profiled different sets of soluble factors whose secretion not only responds to NOTCH activation but also shows differential ligand specificity, suggesting that ligand-specific inputs may regulate the expression of secreted proteins involved in different cardiac development processes. NOTCH signaling activation correlates

SUBMITTER: Torregrosa-Carrion R 

PROVIDER: S-EPMC6731085 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature