Generation of human hepatic progenitor cells with regenerative and metabolic capacities from primary hepatocytes.

ABSTRACT: Hepatocytes are regarded as the only effective cell source for cell transplantation to treat liver diseases; however, their availability is limited due to a donor shortage. Thus, a novel cell source must be developed. We recently reported that mature rodent hepatocytes can be reprogrammed into progenitor-like cells with a repopulative capacity using small molecule inhibitors. Here, we demonstrate that hepatic progenitor cells can be obtained from human infant hepatocytes using the same strategy. These cells, named human chemically induced liver progenitors (hCLiPs), had a significant repopulative capacity in injured mouse livers following transplantation. hCLiPs redifferentiated into mature hepatocytes in vitro upon treatment with hepatic maturation-inducing factors. These redifferentiated

SUBMITTER: Katsuda T 

PROVIDER: S-EPMC6731094 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature