Continuous artificial synthesis of glucose precursor using enzyme-immobilized microfluidic reactors.

ABSTRACT: Food production in green crops is severely limited by low activity and poor specificity of D-ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) in natural photosynthesis (NPS). This work presents a scientific solution to overcome this problem by immobilizing RuBisCO into a microfluidic reactor, which demonstrates a continuous production of glucose precursor at 13.8 μmol g^-1 RuBisCO min^-1 from CO₂ and ribulose-1,5-bisphosphate. Experiments show that the RuBisCO immobilization significantly enhances enzyme stabilities (7.2 folds in storage stability, 6.7 folds in thermal stability), and also improves the reusability (90.4% activity retained after 5 cycles of reuse and 78.5% after 10 cycles). This work mimics the NPS pathway with scalable microreactors

SUBMITTER: Zhu Y 

PROVIDER: S-EPMC6731257 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature