Spatially clustered loci with multiple enhancers are frequent targets of HIV-1 integration.

ABSTRACT: HIV-1 recurrently targets active genes and integrates in the proximity of the nuclear pore compartment in CD4⁺ T cells. However, the genomic features of these genes and the relevance of their transcriptional activity for HIV-1 integration have so far remained unclear. Here we show that recurrently targeted genes are proximal to super-enhancer genomic elements and that they cluster in specific spatial compartments of the T cell nucleus. We further show that these gene clusters acquire their location during the activation of T cells. The clustering of these genes along with their transcriptional activity are the major determinants of HIV-1 integration in T cells. Our results provide evidence of the relevance of the spatial compartmentalization of the genome for HIV-1 integration,

SUBMITTER: Lucic B 

PROVIDER: S-EPMC6731298 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature