Sexual dimorphism in PAR₂-dependent regulation of primitive colonic cells.

ABSTRACT:

Background

Sexual dimorphism in biological responses is a critical knowledge for therapeutic proposals. However, gender differences in intestinal stem cell physiology have been poorly studied. Given the important role of the protease-activated receptor PAR₂ in the control of colon epithelial primitive cells and cell cycle genes, we have performed a sex-based comparison of its expression and of the effects of PAR₂ activation or knockout on cell proliferation and survival functions.

Methods

Epithelial primitive cells isolated from colons from male and female mice were cultured as colonoids, and their number and size were measured. PAR₂ activation was triggered by the addition of SLIGRL agonist peptide in the culture medium. PAR₂-defi

SUBMITTER: Noguerol J 

PROVIDER: S-EPMC6731565 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature