Nano-graphene oxide improved the antibacterial property of antisense yycG RNA on Staphylococcus aureus.

ABSTRACT:

Background

Staphylococcus aureus (S. aureus) has the potential to opportunistically cause infectious diseases, including osteomyelitis, skin infections, pneumonia, and diarrhea. We previously reported that ASyycG RNA reduced the transcripts of virulent genes, and biofilm formation of S. aureus. Currently, graphene oxide (GO) nanosheets are used to efficiently deliver nucleic acids with favorable biocompatibility.

Methods

In the current study, a GO-based recombinant pDL278 ASyycG vector transformation strategy was developed. The particle size distributions and zeta-potential of the GO-PEI-based ASyycG were evaluated. The ASyycG plasmids were labeled with gene-encoding enhanced green fluorescent protein (ASyycG-eGFP). Quantitative real-time PCR assays were performed to investi

SUBMITTER: Wu S 

PROVIDER: S-EPMC6731568 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature