Novel polymerase spiral reaction assay for the visible molecular detection of porcine circovirus type 3.

ABSTRACT:

Background

Porcine circovirus type 3 (PCV3) is a newly emerging circovirus that might be associated with porcine dermatitis and nephropathy syndrome, reproductive failure, and cardiac and multisystemic inflammation. To aid the prevention and control of the infectious disease caused by PCV3, we developed a novel isothermal amplification assay using polymerase spiral reaction (PSR), which allows the visual detection of preserved strains and clinical samples.

Results

This assay precisely amplified the PCV3 genome with the use of a water bath at 62 °C for 50 min. The detection limit was found to be 1.13 × 10² copies/μL by gel electrophoresis or with the use of a visible dye (an indicator comprising phenol red and cresol red). No cross-reaction with other porcine infec

SUBMITTER: Ji J 

PROVIDER: S-EPMC6731610 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature