MiR-145 supports cancer cell survival and shows association with DDR genes, methylation pattern, and epithelial to mesenchymal transition.

ABSTRACT:

Background

Despite several reports describing the dual role of miR-145 as an oncogene and a tumor suppressor in cancer, not much has been resolved and understood.

Method

In this study, the potential targets of miR-145 were identified bio-informatically using different target prediction tools. The identified target genes were validated in vitro by dual luciferase assay. Wound healing and soft agar colony assay assessed cell proliferation and migration. miR-145 expression level was measured quantitatively by RT-PCR at different stages of breast tumor. Western blot was used to verify the role of miR-145 in EMT transition using key marker proteins.

Result

Wound healing and soft agar colony assays, using miR-145 over-expressing stably transfected MCF7 cells, unraveled its

SUBMITTER: Manvati S 

PROVIDER: S-EPMC6731614 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature