Peroxidase evolution in white-rot fungi follows wood lignin evolution in plants.

ABSTRACT: A comparison of sequenced Agaricomycotina genomes suggests that efficient degradation of wood lignin was associated with the appearance of secreted peroxidases with a solvent-exposed catalytic tryptophan. This hypothesis is experimentally demonstrated here by resurrecting ancestral fungal peroxidases, after sequence reconstruction from genomes of extant white-rot Polyporales, and evaluating their oxidative attack on the lignin polymer by state-of-the-art analytical techniques. Rapid stopped-flow estimation of the transient-state constants for the 2 successive one-electron transfers from lignin to the peroxide-activated enzyme (k _2app and k _3app ) showed a progressive increase during peroxidase evolution (up to 50-fold higher values for the

SUBMITTER: Ayuso-Fernandez I 

PROVIDER: S-EPMC6731631 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature