Tetrodotoxin-Sensitive Sodium Channels Mediate Action Potential Firing and Excitability in Menthol-Sensitive Vglut3-Lineage Sensory Neurons.

ABSTRACT: Small-diameter vesicular glutamate transporter 3-lineage (Vglut3^lineage) dorsal root ganglion (DRG) neurons play an important role in mechanosensation and thermal hypersensitivity; however, little is known about their intrinsic electrical properties. We therefore set out to investigate mechanisms of excitability within this population. Calcium microfluorimetry analysis of male and female mouse DRG neurons demonstrated that the cooling compound menthol selectively activates a subset of Vglut3^lineage neurons. Whole-cell recordings showed that small-diameter Vglut3^lineage DRG neurons fire menthol-evoked action potentials and exhibited robust, transient receptor potential melastatin 8 (TRPM8)-dependent discharges at room temperature. This heightened excitabilit

SUBMITTER: Griffith TN 

PROVIDER: S-EPMC6733551 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature