Isoform specific editing of the coxsackievirus and adenovirus receptor.

ABSTRACT: The Coxsackievirus and adenovirus receptor (CAR) is both a viral receptor and cell adhesion protein. CAR has two transmembrane isoforms that localize distinctly in polarized epithelial cells. Whereas the seven exon-encoded isoform (CAR^Ex7) exhibits basolateral localization, the eight exon-encoded isoform (CAR^Ex8) can localize to the apical epithelial surface where it can mediate luminal adenovirus infection. To further understand the distinct biological functions of these two isoforms, CRISPR/Cas9 genomic editing was used to specifically delete the eighth exon of the CXADR gene in a Madine Darby Canine Kidney (MDCK) cell line with a stably integrated lentiviral doxycycline-inducible CAR^Ex8 cDNA. The gene-edited clone demonstrated a significant reduction in

SUBMITTER: Readler JM 

PROVIDER: S-EPMC6733617 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature