The importance of the nuclear positioning of the PPARG gene for its expression during porcine in vitro adipogenesis.

ABSTRACT: Proper expression of the PPARG gene, which encodes a key transcription factor of adipogenesis, is indispensable in the formation of mature adipocytes. The positioning of a gene within the nuclear space has been implicated in gene regulation. We here report on the significance of the PPARG gene's nuclear positioning for its activity during in vitro adipogenesis in the pig. We used an established system of differentiation of mesenchymal stem cells derived from bone marrow and adipose tissue into adipocytes. The differentiation process was carried out for 7 days, and the cells were examined using the 3D DNA/immuno-FISH and RNA/DNA-FISH approaches. PPARG transcript level was measured using real-time PCR, and PPARγ activity was detected with colorimetric assay. Changes in the nuclear location o

SUBMITTER: Stachecka J 

PROVIDER: S-EPMC6733831 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature