Silver ions promote blebs growth in U251 glioblastoma cell by activating nonselective cationic currents.

ABSTRACT: Glioblastoma (GBM) is the most common and aggressive human brain cancer with low prognosis and therefore the discovery of new anticancer agents is needful. Sulfydryl reagents, such as silver, have been shown to induce membrane vesiculation in several cellular models through a mechanism that has not been yet completely clarified. Using U251 glioblastoma cells, we observed that silver induced irreversible bleb formation of the plasma membrane. This morphological event was anticipated by an increase of intracellular Ca²⁺ associated to extracellular Ca²⁺ influx. Accordingly, using patch-clamp whole cell recording during silver ion application, inward current/s (IAg) at -90 mV were detected and cells were permeable to Ca²⁺ and monovalent ions such as Na⁺

SUBMITTER: Ragonese F 

PROVIDER: S-EPMC6733836 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature