The role of Na_V channels in synaptic transmission after axotomy in a microfluidic culture platform.

ABSTRACT: Voltage gated sodium channels are key players in aberrant pain signaling and sensitization of nociceptors after peripheral nerve injury. The extent to which sodium channel activity after injury contributes to synaptic transmission at the first pain synapse however remains unclear. To investigate the effect of axotomy on synaptic transmission between dorsal root ganglia neurons and dorsal horn neurons, we reconstructed the first pain synapse in a novel microfluidic based compartmentalized cell culture system, which recapitulates the connectivity of peripheral pain signaling. We show that following axotomy of the distal axons, inhibition of Na_V1.7 and Na_V1.8 sodium channels in incoming presynaptic DRG axons is no longer sufficient to block activation of these axons and

SUBMITTER: Vysokov N 

PROVIDER: S-EPMC6733904 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature