Benchmarking variant identification tools for plant diversity discovery.

ABSTRACT:

Background

The ability to accurately and comprehensively identify genomic variations is critical for plant studies utilizing high-throughput sequencing. Most bioinformatics tools for processing next-generation sequencing data were originally developed and tested in human studies, raising questions as to their efficacy for plant research. A detailed evaluation of the entire variant calling pipeline, including alignment, variant calling, variant filtering, and imputation was performed on different programs using both simulated and real plant genomic datasets.

Results

A comparison of SOAP2, Bowtie2, and BWA-MEM found that BWA-MEM was consistently able to align the most reads with high accuracy, whereas Bowtie2 had the highest overall accuracy. Comparative results of GATK Haplot

SUBMITTER: Wu X 

PROVIDER: S-EPMC6734213 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature