Allosteric Inhibitor of KRas Identified Using a Barcoded Assay Microchip Platform.

ABSTRACT: Protein catalyzed capture agents (PCCs) are synthetic antibody surrogates that can target a wide variety of biologically relevant proteins. As a step toward developing a high-throughput PCC pipeline, we report on the preparation of a barcoded rapid assay platform for the analysis of hits from PCC library screens. The platform is constructed by first surface patterning a micrometer scale barcode composed of orthogonal ssDNA strands onto a glass slide. The slide is then partitioned into microwells, each of which contains multiple copies of the full barcode. Biotinylated candidate PCCs from a click screen are assembled onto the barcode stripes using a complementary ssDNA-encoded cysteine-modified streptavidin library. This platform was employed to evaluate candidate PCC ligands identified fro

SUBMITTER: McCarthy AM 

PROVIDER: S-EPMC6734936 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature