Small-Animal PET/CT Imaging of Local and Systemic Immune Response Using ⁶⁴Cu-αCD11b.

ABSTRACT: Current noninvasive imaging methods for monitoring immune response were largely developed for interrogation of the local reaction. This study developed the radiotracer ⁶⁴Cu-labeled anti-CD11b (⁶⁴Cu-αCD11b) for longitudinal assessment of local and systemic immune response involving mobilization of CD11b⁺ myeloid cells by small-animal PET/CT. Methods: Acute or chronic inflammation in the ears of BALB/c mice was induced by 12-o-tetradecanoylphorbol-13-acetate. Acute lung inflammation was induced by intratracheal lipopolysaccharide inoculation. αCD11b was conjugated with p-SCN-Bn-DOTA followed by labeling with ⁶⁴Cu. PET/CT and biodistribution were evaluated at different times after intravenous injection of ⁶⁴Cu-αCD11b.

SUBMITTER: Cao Q 

PROVIDER: S-EPMC6735280 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature