Weaving DNA strands: structural insight on ATP hydrolysis in RecA-induced homologous recombination.

ABSTRACT: Homologous recombination is a fundamental process in all living organisms that allows the faithful repair of DNA double strand breaks, through the exchange of DNA strands between homologous regions of the genome. Results of three decades of investigation and recent fruitful observations have unveiled key elements of the reaction mechanism, which proceeds along nucleofilaments of recombinase proteins of the RecA family. Yet, one essential aspect of homologous recombination has largely been overlooked when deciphering the mechanism: while ATP is hydrolyzed in large quantity during the process, how exactly hydrolysis influences the DNA strand exchange reaction at the structural level remains to be elucidated. In this study, we build on a previous geometrical approach that studied the RecA fil

SUBMITTER: Boyer B 

PROVIDER: S-EPMC6735932 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature