Using Microarrays to Interrogate Microenvironmental Impact on Cellular Phenotypes in Cancer.

ABSTRACT: Understanding the impact of the microenvironment on the phenotype of cells is a difficult problem due to the complex mixture of both soluble growth factors and matrix-associated proteins in the microenvironment in vivo. Furthermore, readily available reagents for the modeling of microenvironments in vitro typically utilize complex mixtures of proteins that are incompletely defined and suffer from batch to batch variability. The microenvironment microarray (MEMA) platform allows for the assessment of thousands of simple combinations of microenvironment proteins for their impact on cellular phenotypes in a single assay. The MEMAs are prepared in well plates, which allows the addition of individual ligands to separate wells containing arrayed extracellular matrix (ECM) proteins. The combinati

SUBMITTER: Smith R 

PROVIDER: S-EPMC6736605 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature