MiRNA-dependent regulation of STIM1 expression in breast cancer.

ABSTRACT: Store-operated Ca²⁺ entry (SOCE) has been shown to be important for breast cancer metastasis in xenograft mouse models. The ER Ca²⁺ sensor STIM1 and Orai plasma membrane Ca²⁺ channels molecularly mediate SOCE. Here we investigate the role of the microRNA machinery in regulating STIM1 expression. We show that STIM1 expression is regulated post-transcriptionally by the miRNA machinery and identify miR-223 and miR-150 as regulators of STIM1 expression in the luminal non-aggressive MCF7 breast cancer cell line. In contrast, STIM1 expression in the more aggressive basal triple-negative MDA-MB-231 cell line is not significantly modulated by a single miRNA species but is rather upregulated due to inhibition of the miRNA machinery through downregulation of Ago2. Co

SUBMITTER: Kulkarni RP 

PROVIDER: S-EPMC6736934 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature