Reinvigoration of diploid strawberry (Fragaria vesca) during adventitious shoot regeneration.

ABSTRACT: Diploid strawberry (Fragaria vesca 'Baiguo') is a model plant for studying functional genomics in Rosaceae. Adventitious shoot regeneration is essential for functional genomics by Agrobacterium tumefaciens-mediated transformation. An efficient shoot regeneration method using diploid strawberry leaf explants was conducted on 1/2MS + 1/2B₅ medium that contained 2.0 mg L^-1 TDZ over 14 days of dark culture; this induced the maximum percentage of shoot regeneration (96.44 ± 1.60%) and the highest number of shoots per explant (23.46 ± 2.14) after 11 weeks of culture. The explants considerably enlarged after 12 days; then, turned greenish brown after 30 days, yellowish brown after 36 days, and completely brown and necrotic after 48 days. Large numbers of adventitious shoots

SUBMITTER: Wang H 

PROVIDER: S-EPMC6736952 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature