Non-invasive Potential Circulating mRNA Markers for Colorectal Adenoma Using Targeted Sequencing.

ABSTRACT: We have developed an optimized protocol for plasma targeted mRNA sequencing in our previous study. Here, we performed plasma targeted mRNA sequencing for 40 colorectal adenoma patients and 39 colonoscopy-proven normal controls in order to find potential circulating mRNA markers for colorectal adenoma. Results showed that GSK3A and RHOA were differential expressed genes identified by a cut-off of fold change >2 and adjusted P value < 0.05. More detailed analysis showed that the expression of both GSK3A (0.01-fold with adjusted P < 1 × 10^-6) and RHOA (0.35-fold with adjusted P < 0.01) in adenoma patients was significantly lower than those in normal healthy subjects. Based on the enrichment analysis of biological process for potential markers, we found that the regulation of progra

SUBMITTER: Xue VW 

PROVIDER: S-EPMC6736954 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature