De novo synthesis of serine and glycine fuels purine nucleotide biosynthesis in human lung cancer tissues.

ABSTRACT: Nucleotide synthesis is essential to proliferating cells, but the preferred precursors for de novo biosynthesis are not defined in human cancer tissues. We have employed multiplexed stable isotope-resolved metabolomics to track the metabolism of [¹³C₆]glucose, D₂-glycine, [¹³C₂]glycine, and D₃-serine into purine nucleotides in freshly resected cancerous and matched noncancerous lung tissues from nonsmall cell lung cancer (NSCLC) patients, and we compared the metabolism with established NSCLC PC9 and A549 cell lines in vitro Surprisingly, [¹³C₆]glucose was the best carbon source for purine synthesis in human NSCLC tissues, in contrast to the noncancerous lung tissues from the same patient,

SUBMITTER: Fan TWM 

PROVIDER: S-EPMC6737211 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature