The ability of salts to stabilize proteins in vivo or intracellularly correlates with the Hofmeister series of ions.

ABSTRACT: Numerous studies have been conducted on the ability of salts to stabilize proteins in vitro using purified proteins demonstrating the fact that the ability of salts to stabilize proteins correlates with the Hofmeister series of ions. Using the well characterized bacterial aqueous cytosolic β-galactosidase and catechol 2,3-dioxygenase enzymes, we demonstrated that salts can stabilize proteins in vivo or intracellularly as well and that the ability of salts to stabilize these two proteins intracellularly also correlates with the Hofmeister series of ions. Na₂SO₄ and Na₂HPO₄ were very effective at stabilizing both proteins, followed by NaCl, NH₄Cl and (NH₄)₂HPO₄, while NH₄CH_3<

SUBMITTER: Hani FM 

PROVIDER: S-EPMC6737386 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature