Engineered Thermoanaerobacterium aotearoense with nfnAB knockout for improved hydrogen production from lignocellulose hydrolysates.

ABSTRACT:

Background

As a renewable and clean energy carrier, the production of biohydrogen from low-value feedstock such as lignocellulose has increasingly garnered interest. The NADH-dependent reduced ferredoxin:NADP⁺ oxidoreductase (NfnAB) complex catalyzes electron transfer between reduced ferredoxin and NAD(P)⁺, which is critical for production of NAD(P)H-dependent products such as hydrogen and ethanol. In this study, the effects on end-product formation of deletion of nfnAB from Thermoanaerobacterium aotearoense SCUT27 were investigated.

Results

Compared with the parental strain, the NADH/NAD⁺ ratio in the ∆nfnAB mutant was increased. The concentration of hydrogen and ethanol produced increased by (41.1 ± 2.37)% (p < 0

SUBMITTER: Li Y 

PROVIDER: S-EPMC6737674 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature