High-speed multifocal plane fluorescence microscopy for three-dimensional visualisation of beating flagella.

ABSTRACT: Analysis of flagellum and cilium beating in three dimensions (3D) is important for understanding cell motility, and using fluorescence microscopy to do so would be extremely powerful. Here, high-speed multifocal plane fluorescence microscopy, where the light path is split to visualise multiple focal planes simultaneously, was used to reconstruct Trypanosoma brucei and Leishmania mexicana movement in 3D. These species are uniflagellate unicellular parasites for which motility is vital. It was possible to use either a fluorescent stain or a genetically-encoded fluorescent protein to visualise flagellum and cell movement at 200 Hz frame rates. This addressed two open questions regarding Trypanosoma and Leishmania flagellum beating, which contributes to their swimmi

SUBMITTER: Walker BJ 

PROVIDER: S-EPMC6737910 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature