Myosin V regulates synaptopodin clustering and localization in the dendrites of hippocampal neurons.

ABSTRACT: The spine apparatus (SA) is an endoplasmic reticulum-related organelle that is present in a subset of dendritic spines in cortical and pyramidal neurons, and plays an important role in Ca²⁺ homeostasis and dendritic spine plasticity. The protein synaptopodin is essential for the formation of the SA and is widely used as a maker for this organelle. However, it is still unclear which factors contribute to its localization at selected synapses, and how it triggers local SA formation. In this study, we characterized development, localization and mobility of synaptopodin clusters in hippocampal primary neurons, as well as the molecular dynamics within these clusters. Interestingly, synaptopodin at the shaft-associated clusters is less dynamic than at spinous clusters. We identify the

SUBMITTER: Konietzny A 

PROVIDER: S-EPMC6737913 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature