Multi-modal and multiscale imaging approaches reveal novel cardiovascular pathophysiology in Drosophila melanogaster.

ABSTRACT: Establishing connections between changes in linear DNA sequences and complex downstream mesoscopic pathology remains a major challenge in biology. Herein, we report a novel, multi-modal and multiscale imaging approach for comprehensive assessment of cardiovascular physiology in Drosophila melanogaster We employed high-speed angiography, optical coherence tomography (OCT) and confocal microscopy to reveal functional and structural abnormalities in the hdp² mutant, pre-pupal heart tube and aorta relative to controls. hdp² harbor a mutation in wupA, which encodes an ortholog of human troponin I (TNNI3). TNNI3 variants frequently engender cardiomyopathy. We demonstrate that the hdp² aortic and cardiac muscle wall

SUBMITTER: Weismann CG 

PROVIDER: S-EPMC6737974 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature