Notch2 pathway mediates breast cancer cellular dormancy and mobilisation in bone and contributes to haematopoietic stem cell mimicry.

ABSTRACT:

Background

Recurrence after >5-year disease-free survival affects one-fifth of breast cancer patients and is the clinical manifestation of cancer cell reactivation after persistent dormancy.

Methods

We investigated cellular dormancy in vitro and in vivo using breast cancer cell lines and cell and molecular biology techniques.

Results

We demonstrated cellular dormancy in breast cancer bone metastasis, associated with haematopoietic stem cell (HSC) mimicry, in vivo competition for HSC engraftment and non-random distribution of dormant cells at the endosteal niche. Notch2 signal implication was demonstrated by immunophenotyping the endosteal niche-associated cancer cells and upon co-culture with sorted endosteal niche cells, which inhibited breast cancer cell proliferati

SUBMITTER: Capulli M 

PROVIDER: S-EPMC6738045 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature