Inhibition of de novo ceramide biosynthesis affects aging phenotype in an in vitro model of neuronal senescence.

ABSTRACT: Although aging is considered to be an unavoidable event, recent experimental evidence suggests that the process can be counteracted. Intracellular calcium (Ca²⁺_i) dyshomeostasis, mitochondrial dysfunction, oxidative stress, and lipid dysregulation are critical factors that contribute to senescence-related processes. Ceramides, a pleiotropic class of sphingolipids, are important mediators of cellular senescence, but their role in neuronal aging is still largely unexplored. In this study, we investigated the effects of L-cycloserine (L-CS), an inhibitor of thede novoceramide biosynthesis, on the aging phenotype of cortical neurons cultured for 22 days, a setting employed as anin vitromodel of senescence. Our findings indicate that, compared to control cult

SUBMITTER: Granzotto A 

PROVIDER: S-EPMC6738398 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature