Characterization of doxycycline-dependent inducible Simian Virus 40 large T antigen immortalized human conjunctival epithelial cell line.

ABSTRACT:

Purpose

To present the properties of a newly developed immortalized human conjunctival epithelial cell (iHCjEC) line.

Methods

iHCjECs were developed to induce Simian Virus 40 large T-antigen (SV40LT) by incorporating lentivirus in a tetracycline (Tet)-regulated gene-expression system into primary cultures of human conjunctival epithelial cells. The population doubling time and morphology of the iHCjECs were analyzed. The expressions of CK13, CK19, CK12, and MUC1, MUC4, MUC16, and MUC5AC were determined by real time PCR and immunohistochemically under different culture conditions. The organotypic culture model in which iHCjECs were cultured on rabbit conjunctival fibroblast-embedded collagen gel was used to characterize the iHCjECs.

Results

The iHCjECs cultured with do

SUBMITTER: Mitani A 

PROVIDER: S-EPMC6738650 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature